Analysis of Phosphate Essay

The aim of the experiment is to determine the concentration of phosphate ions in unknown samples. A series of dilution of known concentration is performed from the phosphate working solution. Each of the prepared working standards, the unknown samples and the blank are then made to react with a mixed reagent which have been prepared after acid wash of the glasswares Glassware * 2 Beakers * 11 conical flasks * 9 Volumetric flasks 50 ml * 1 Volumetric flasks 1000 ml * Pipette type 1 Pipette 25 ml * Stir rod * 11cuvette * Spectrophotometer Chemical needs; hazards and safety precautions: 1.

Sulphuric acid It is very hazardous in case of skin contact. It is classified as corrosive, irritant and permeator. Safety needs: Lab coats,safety goggles and gloves should be worn to minimize risk of contact. In case of: •Eye Contact: Check for and remove any contact lenses. In case of contact, immediately flush eyes with plenty of water for at least 15minutes. Cold water may be used. Get medical attention immediately. •Skin Contact:

Don't use plagiarized sources. Get Your Custom Essay on
Analysis of Phosphate Essay
From as low as $9/Page
Order Essay

In case of contact, immediately flush skin with plenty of water for at least 15 minutes while removing contaminated clothing and shoes.

Cover the irritated skin with an emollient. Cold water may be used. Wash clothing before reuse. Thoroughly clean shoes before reuse. Get medical attention immediately. •Serious Skin Contact: Wash with a disinfectant soap and cover the contaminated skin with an anti-bacterial cream. Seek immediate medical attention. •Inhalation: If inhaled, remove to fresh air. If not breathing, give artificial respiration. If breathing is difficult, give oxygen.

Get medical attention immediately. 2. ammonium heptimolybdate Ammonium heptimolybdate e is harmful if swallowed or inhaled. It causes irritation to the eyes, skin, and respiratory tract. It affects kidneys and blood Safety measures: Lab coats,safety goggles and gloves should be worn to minimize risk of contact. 3. Potassium antimonyl tartrate Slightly hazardous in case of ingestion. Non-corrosive for skin. Non-sensitizer for skin. 4. Ascorbic acid Slightly hazardous in case of skin contact (irritant), of eye contact (irritant), of ingestion, of inhalation.

Safety measures: Lab coats, safety goggles and gloves should be worn to minimize risk of contact. 5. Hydrochloric acid Concentrated hydrochloric acid forms acidic mists. Both the mist and the solution have a corrosive effect on human tissue, with the potential to damage respiratory organs, eyes, skin, and intestines. The HCL should be used in a fume hood and personal protective equipment such as rubber or PVC gloves, protective eye goggles, and chemical-resistant clothing such as lab coats should be used to minimize risk when handling hydrochloric acid.

Procedures and calculations. * All of the glassware’s to be used are to be washed with acid (HCL) and then with distilled water. * Nine standards, a blank and 2 random sample are going to be analysed. * Prepare a series of working standards in the range of 10-500µg NO2-N/L * To 50 cm3 of each working standard and sample add 1. 0 cm3 of sulphanilamide, mix and stand for 2 minutes Calculations 1ml = 0. 5 µg of NO2- 1000 ml of solution contain = 10 ml of NO2- (100 µg) 50 ml of solution contain = 1 ml of NO2- (10 µg)

SN| Volume of standardPHOSPHATE solution (s)| Volume ofwater| Concentration| Absorbance| 1. | 1. 0| 49| 10| 0. 003| 2. | 2. 0| 45| 50| 0. 035| 3. | 3. 0| 40| 100| 0. 058| 4. | 4. 0| 35| 150| 0. 074| 5. | 5. 0| 30| 200| 0. 099| Blank| 0| 50| 0| 0. 000| Sample A1| | 0. 022| Sample B1| | 0. 068| Result A Graph of absorbance (OD) against concentration (µg). Using the equation (y=mx+b) to determine concentration of samples. Let “Y” equal the concentration. This is what will be solved for. Let “X” equal the absorbency of the sample.

This is the absorbency measured by the spectrophotometer Allow “to equal the slope and “b” to equal the y-intercept y= 0. 019 x + 0. 947 Concentration of Sample 1 Absorbance of sample A1 = 0. 022 y= 0. 019 (0. 022) + 0. 947 = 0. 947 Concentration of unknown sample 2 Absorbance of sample B2 = 0. 068 y= 0. 019 (0. 068) + 0. 947 = 0. 948 Discussion The concentration phosphate ion in of samples D and E were determined In this experiment I determined the concentration of phosphate ion in samples D and E.

These values were determined by obtaining data from solutions of known concentration. These solutions were placed in the spectrophotometer and then by plotting a graph of absorbance vs. concentration, an equation was produced from which the unknown concentration were determined Conclusion From the result of the experiment, it was determined that the relationship between concentration and absorbance is linear. The amount of light absorbed by a solution varies directly with solute concentration.

The Homework Writings
Order NOW For A 10% Discount!
Pages (550 words)
Approximate price: -

Our Advantages

Plagiarism Free Papers

All our papers are original and written from scratch. We will email you a plagiarism report alongside your completed paper once done.

Free Revisions

All papers are submitted ahead of time. We do this to allow you time to point out any area you would need revision on, and help you for free.

Title-page

A title page preceeds all your paper content. Here, you put all your personal information and this we give out for free.

Bibliography

Without a reference/bibliography page, any academic paper is incomplete and doesnt qualify for grading. We also offer this for free.

Originality & Security

At thehomeworkwritings.com, we take confidentiality seriously and all your personal information is stored safely and do not share it with third parties for any reasons whatsoever. Our work is original and we send plagiarism reports alongside every paper.

24/7 Customer Support

Our agents are online 24/7. Feel free to contact us through email or talk to our live agents.

Try it now!

Calculate the price of your order

We'll send you the first draft for approval by at
Total price:
$0.00

How it works?

Follow these simple steps to get your paper done

Place your order

Fill in the order form and provide all details of your assignment.

Proceed with the payment

Choose the payment system that suits you most.

Receive the final file

Once your paper is ready, we will email it to you.

Our Services

We work around the clock to see best customer experience.

Pricing

Flexible Pricing

Our prices are pocket friendly and you can do partial payments. When that is not enough, we have a free enquiry service.

Communication

Admission help & Client-Writer Contact

When you need to elaborate something further to your writer, we provide that button.

Deadlines

Paper Submission

We take deadlines seriously and our papers are submitted ahead of time. We are happy to assist you in case of any adjustments needed.

Reviews

Customer Feedback

Your feedback, good or bad is of great concern to us and we take it very seriously. We are, therefore, constantly adjusting our policies to ensure best customer/writer experience.